SNRPN antibody

Catalog No. ABIN5704718

The Rabbit Polyclonal anti-SNRPN antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect SNRPN in samples from Human, Mouse and Rat.

Quick Overview for SNRPN antibody (ABIN5704718)

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRPN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-SNRPN Antibody

Immunogen: survival of motor neuron 2, centromeric

Isotype: IgG

Application Details

Application Notes: WB : 1:500-1:5000 IP : 1:500-1:5000 IHC : 1:50-1:200 IF : 1:10-1:100

Comment:

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab08030(SMN2 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for SNRPN

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Alternative Name: SMN

Background: Synonyms:C BCD541, Component of gems 1, FLJ76644, Gemin 1, SMN, SMN1, SMN1, SMN, SMN2, SMNC, SMNT, Survival motor neuron protein Background:This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. While mutations in the telomeric copy are associated with spinal muscular atrophy, mutations in this gene, the centromeric copy, do not lead to disease. This gene may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7, which is thought to be an exon splice enhancer. Note that the nine exons of both the telomeric and centromeric copies are designated historically as exon 1, 2a, 2b, and 3-8. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The full length protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein. Four transcript variants encoding distinct isoforms have been described.

UniProt: Q16637