CHN2 antibody (C-Term)

Catalog No. ABIN570662

The Goat Polyclonal anti-CHN2 antibody has been validated for ELISA and IHC. It is suitable to detect CHN2 in samples from Human.

Quick Overview for CHN2 antibody (C-Term) (ABIN570662)

Target: CHN2 (Chimerin (Chimaerin) 2 (CHN2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHN2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CHN2 Antibody

Binding Specificity: C-Term

Purpose: CHN2

Sequence: QILIENEDVL F

Specificity: This antibody is expected to recognize both reported isoforms (NP_004058.1, NP_001035025.1).

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QILIENEDVLF, from the C Terminus of the protein sequence according to NP_001035025.1, NP_004058.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cortex shows staining of neuronal bodies. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments in Human Brain (Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHN2

Target: CHN2 (Chimerin (Chimaerin) 2 (CHN2))

Alternative Name: CHN2

Background: CHN2, chimerin (chimaerin) 2, ARHGAP3, RHOGAP3, RhoGAP3, beta chimerin, Chimerin 2 (GTPase-activating protein, rho, 3), BCH, MGC138360, rho-GTPase-activating protein 3

Gene ID: 1124

NCBI Accession: NP_001035025, NP_004058