Histone H4 antibody (meLys20)

Catalog No. ABIN5706797

Supplier Product No.: 600-401-j01

The Rabbit Polyclonal anti-Histone H4 antibody has been validated for WB, IHC, IF, ChIP, DB, MA and FM. It is suitable to detect Histone H4 in samples from Human, Mouse and C. elegans. There is 1 publication available.

Quick Overview for Histone H4 antibody (meLys20) (ABIN5706797)

Target: Histone H4

Reactivity: Human, Mouse, C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Product Details anti-Histone H4 Antibody

Binding Specificity: meLys20

Supplier Product No.: 600-401-j01

Supplier: Rockland

Purpose: Histone H4 K20me1 Antibody

Cross-Reactivity (Details): This antibody reacts with human Histone H4.

Characteristics: Synonyms: rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Purification: Anti-Histone H4 [Monomethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H4 [Monomethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H4 [Monomethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:50

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.12 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Histone H4 antibody (ABIN5706797)

Moiseeva, Hood, Schamus, OConnor, Conrads, Bakkenist: "ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).

Target Details for Histone H4

Target: Histone H4

Background: Background: SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.

Gene ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805