Histone H4 antibody (meArg3)

Catalog No. ABIN5706801

Supplier Product No.: 600-401-i97

This anti-Histone H4 antibody is a Rabbit Polyclonal antibody detecting Histone H4 in WB, IHC, IF, ChIP and FM. Suitable for Human and Mouse.

Quick Overview for Histone H4 antibody (meArg3) (ABIN5706801)

Target: Histone H4

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Product Details anti-Histone H4 Antibody

Binding Specificity: meArg3

Supplier Product No.: 600-401-i97

Supplier: Rockland

Purpose: Histone H4 R3me1 Antibody

Cross-Reactivity (Details): This antibody reacts with human Histone H4.

Characteristics: Synonyms: rabbit anti-Histone H4 monomethyl Arg3 antibody, H4R3me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Purification: Anti-Histone H4 [Monomethyl Arg3] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H4 [Monomethyl Arg3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50

Application Note: Anti-Histone H4 [Monomethyl Arg3] antibody is tested for Western Blot and Immunofluorescence. This antibody is useful in Dot Blot, Chromatin Immunoprecipitation, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:50

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.43 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone H4

Target: Histone H4

Background: Background: Histone H4 is an 11.4 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. Histone H4 shows transcriptional regulation that is both cell cycle-dependent and -independent. Histone H4 is modified by phosphorylation, acetylation, ribosylation, and methylation. Histone H4 has been shown to interact with other histone acetytransferases, histone deacetylases, and PARP. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805