HUWE1 antibody (Internal Region)

Catalog No. ABIN570775

Product Details anti-HUWE1 Antibody

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HUWE1 antibody is un-conjugated

Application: ELISA

Purpose: HUWE1 / LASU1

Sequence: DDLKSNTEYH KYQ

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Recent

Immunogen: Peptide with sequence C-DDLKSNTEYHKYQ, from the internal region of the protein sequence according to NP_113584.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 482 kDa according to NP_113584.3. Therefore we cannot recommend an optimal concentration and the antibody i

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Alternative Name: HUWE1 (HUWE1 Products)

Background: HUWE1, HECT, UBA and WWE domain containing 1, ARF-BP1, HECTH9, HSPC272, Ib772, KIAA0312, LASU1, MULE, UREB1, ARF binding protein 1, BJ-HCC-24 tumor antigen, HECT domain protein LASU1, Mcl-1 ubiquitin ligase E3, OTTHUMP00000023356, OTTHUMP00000061860

Gene ID: 10075, 59026

NCBI Accession: NP_113584