KRT15 antibody

Catalog No. ABIN5707763

This Rabbit Monoclonal antibody specifically detects KRT15 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for KRT15 antibody (ABIN5707763)

Target: KRT15 (Keratin 15 (KRT15))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRT15-2103R

Product Details anti-KRT15 Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the recombinant Cytokeratin 15 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Cytokeratin 15 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Cytokeratin 15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: KRT15 / Cytokeratin 15

Background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.