ADA antibody (AA 2-241)

Catalog No. ABIN5708188

This anti-ADA antibody is a Rabbit Polyclonal antibody detecting ADA in WB, ELISA and IHC (p). Suitable for Mouse and Rat.

Quick Overview for ADA antibody (AA 2-241) (ABIN5708188)

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADA Antibody

Binding Specificity: AA 2-241

Purification: Antigen affinity purified

Immunogen: A recombinant rat partial protein corresponding to amino acids A2-H241 was used as the immunogen for the Ada antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Ada antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,ELISA (Capture): 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Ada antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: Ada / Adenosine deaminase

Background: Adenosine Deaminase (also known as Adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

UniProt: Q920P6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process