2B4 antibody (Internal Region)

Catalog No. ABIN570901

This Goat Polyclonal antibody specifically detects 2B4 in ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for 2B4 antibody (Internal Region) (ABIN570901)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-2B4 Antibody

Binding Specificity: Internal Region

Purpose: phospho-CD244 / 2B4

Sequence: EFLTIpYEDV KD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EFLTIpYEDVKD, from the internal region of the protein sequence according to NP_057466.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1 kDa according to NP_057466.1). We do note that CD244 is a

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244

Background: CD244, CD244 Molecule, natural killer cell receptor 2B4, 2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 natural killer cell receptor 2B4, NK cell activation inducing ligand NAIL, natural killer cell receptor 2B4

Gene ID: 51744

NCBI Accession: NP_057466