UBE2F antibody (C-Term)

Catalog No. ABIN570941

The Goat Polyclonal anti-UBE2F antibody has been validated for WB, ELISA and IHC. It is suitable to detect UBE2F in samples from Human and Mouse.

Quick Overview for UBE2F antibody (C-Term) (ABIN570941)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-UBE2F Antibody

Binding Specificity: C-Term

Purpose: NCE2 / UBE2F

Sequence: HLRDKEDFRN KVDD

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HLRDKEDFRNKVDD, from the C Terminus of the protein sequence according to NP_542409.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration: 2.5-3.8 μg/mL.

Western Blot: Approx. 20 kDa band observed in Mouse Liver lysates (calculated MW of 21.1 kDa according to Human NP_542409.1 and to Mouse NP_080730.1). Recommended concentration: 0.05-0.2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme

Gene ID: 140739, 67921, 363284

NCBI Accession: NP_542409