FBXO32 antibody (Internal Region)

Catalog No. ABIN570946

This Goat Polyclonal antibody specifically detects FBXO32 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for FBXO32 antibody (Internal Region) (ABIN570946)

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXO32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FBXO32 Antibody

Binding Specificity: Internal Region

Purpose: FBXO32

Sequence: NSKTKTQYFH QEK

Specificity: This antibody is expected to recognize reported isoform 1 (NP_478136.1).

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NSKTKTQYFHQEK, from the internal region of the protein sequence according to NP_478136.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 40 kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 41.5 kDa according to Mouse NP_080622.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessf

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FBXO32

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Alternative Name: FBXO32

Background: FBXO32, F-box protein 32, FLJ32424, Fbx32, MAFbx, MGC33610, F-box only protein 32, atrogin 1, muscle atrophy F-box protein

Gene ID: 114907, 67731, 171043

NCBI Accession: NP_478136