NK2 Homeobox 5 antibody (Internal Region)

Catalog No. ABIN570994

This Goat Polyclonal antibody specifically detects NK2 Homeobox 5 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NK2 Homeobox 5 antibody (Internal Region) (ABIN570994)

Target: NK2 Homeobox 5 (NKX2-5)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NK2 Homeobox 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NK2 Homeobox 5 Antibody

Binding Specificity: Internal Region

Purpose: CSX1 / NKX2-5

Sequence: PRAYSDPDPA KDPR

Specificity: This antibody is expected to recognize all three reported isoforms (NP_004378.1, NP_001159647.1, NP_001159648.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1, NP_001159647.1, NP_001159648.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5-6 μg/mL.

Western Blot: Approx. 38 kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9 kDa according to Human NP_004378.1, 34.2 kDa according to Mouse NP_032726.1 and 34.3 kDa according to Rat NP_446103.2). Recommended concentration: 1-3 μg/m

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NK2 Homeobox 5

Target: NK2 Homeobox 5 (NKX2-5)

Alternative Name: NKX2-5

Background: NKX2-5, NK2 transcription factor related, locus 5 (Drosophila), CHNG5, CSX, CSX1, NKX2.5, NKX2E, NKX4-1, NK2 transcription factor homolog E, NK2 transcription factor related, locus 5, cardiac-specific homeo box, tinman paralog

Gene ID: 1482, 18091, 114109

NCBI Accession: NP_004378, NP_001159647, NP_001159648

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development