SATB1 antibody (Internal Region)

Catalog No. ABIN571004

The Goat Polyclonal anti-SATB1 antibody is suitable to detect SATB1 in samples from Human. It has been validated for WB, IHC, IF, ELISA and FACS.

Quick Overview for SATB1 antibody (Internal Region) (ABIN571004)

Target: SATB1 (SATB Homeobox 1 (SATB1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SATB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SATB1 Antibody

Binding Specificity: Internal Region

Purpose: SATB1

Sequence: ESDEENRQKT RPRT

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell lines A431 and Jurkat and in lysates of cell lines U251 and Daudi (calculated MW of 89.0 kDa according to NP_001182399.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Alternative Name: SATB1

Background: SATB1, SATB homeobox 1, DNA-binding protein SATB1, special AT-rich sequence binding protein 1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)

Gene ID: 6304, 20230, 316164

NCBI Accession: NP_002962

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation