P2RX7 antibody (N-Term)

Catalog No. ABIN571021

This Goat Polyclonal antibody specifically detects P2RX7 in WB, IHC, ELISA, IF. It exhibits reactivity toward Human, Mouse.

Quick Overview for P2RX7 antibody (N-Term) (ABIN571021)

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This P2RX7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-P2RX7 Antibody

Binding Specificity: N-Term

Purpose: P2RX7 / P2X7 receptor

Sequence: YETNKVTRIQ SMNY

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YETNKVTRIQSMNY-C, from the N Terminus of the protein sequence according to NP_002553.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 37 kDa and 70 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6 kDa according to NP_002553.2). Recommended concentration: 1-3 μg/mL. Primary incubation was an 1 hour. An additional band of unknown identity

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Thaler R et al. (2015) PMID: 25491310.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for P2RX7

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Alternative Name: P2RX7

Background: P2RX7, purinergic receptor P2X, ligand-gated ion channel, 7, MGC20089, P2X7, ATP receptor, P2X purinoceptor 7, P2X7 receptor, P2Z receptor, purinergic receptor P2X7

Gene ID: 5027, 18439, 29665

NCBI Accession: NP_002553

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis