TET2 antibody (Internal Region)

Catalog No. ABIN571057

This Goat Polyclonal antibody specifically detects TET2 in IHC, ELISA and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for TET2 antibody (Internal Region) (ABIN571057)

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TET2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TET2 Antibody

Binding Specificity: Internal Region

Purpose: TET2

Sequence: PHPQSNNDQQ REGSF

Specificity: This antibody is expected to recognize both reported isoforms (NP_001120680.1, NP_060098.3).

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-PHPQSNNDQQREGSF

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining in select splenocytes. Recommended concentration: 2-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TET2 antibody (ABIN571057)

Orr, Haffner, Nelson, Yegnasubramanian, Eberhart: "Decreased 5-hydroxymethylcytosine is associated with neural progenitor phenotype in normal brain and shorter survival in malignant glioma." in: PLoS ONE, Vol. 7, Issue 7, pp. e41036, (2012) (PubMed).

Target Details for TET2

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Alternative Name: TET2

Background: TET2, tet oncogene family member 2, FLJ20032, KIAA1546, MGC125715, OTTHUMP00000161869

Gene ID: 54790, 214133

NCBI Accession: NP_001120680, NP_060098

Pathways: Warburg Effect