GAL antibody (Internal Region)

Catalog No. ABIN571062

This anti-GAL antibody is a Goat Polyclonal antibody detecting GAL in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for GAL antibody (Internal Region) (ABIN571062)

Target: GAL (Galanin Prepropeptide (GAL))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GAL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-GAL Antibody

Binding Specificity: Internal Region

Purpose: GAL

Sequence: HRSFSDKNGL TSK

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HRSFSDKNGLTSK, from the internal region of the protein sequence according to NP_057057.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration: 0.2-0.5 μg/mL.

Western Blot: Approx 17 kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 13.3 kDa according to Human NP_057057.2 and of 13.5 kDa according to Mouse NP_034383.1 and Rat NP_150240.1). Recommended concentration

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GAL

Target: GAL (Galanin Prepropeptide (GAL))

Alternative Name: GAL

Background: GAL, galanin prepropeptide, GALN, GLNN, GMAP, MGC40167, galanin, galanin-message-associated peptide, galanin-related peptide

Gene ID: 51083, 14419, 29141

NCBI Accession: NP_057057

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Feeding Behaviour