Tppp antibody (Internal Region)

Catalog No. ABIN571099

This Goat Polyclonal antibody specifically detects Tppp in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Tppp antibody (Internal Region) (ABIN571099)

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Tppp antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Tppp Antibody

Binding Specificity: Internal Region

Purpose: TPPP/p25

Sequence: DPSKDRAAKR LSLES

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DPSKDRAAKRLSLES

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Alternative Name: TPPP

Background: TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein

Gene ID: 11076, 72948, 361466

NCBI Accession: NP_008961