MYB antibody (AA 557-569)

Catalog No. ABIN571166

The Goat Polyclonal anti-MYB antibody has been validated for WB and ELISA. It is suitable to detect MYB in samples from Human.

Quick Overview for MYB antibody (AA 557-569) (ABIN571166)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MYB Antibody

Binding Specificity: AA 557-569

Purpose: c-Myb (aa557-569)

Sequence: HRDQTVKTQK ENT

Specificity: This antibody is expected to recognize all reported isofoms. (NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1, NP_001155132.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HRDQTVKTQKENT, from the internal region of the protein sequence according to NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1, NP_001155132.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 85 kDa band observed in lysates of cell line Jurkat (calculated MW of 85.5 kDa according to NP_001123645.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: MYB

Background: MYB, v-myb myeloblastosis viral oncogene homolog (avian), Cmyb, c-myb, c-myb_CDS, efg, c-myb protein (140 AA), c-myb10A_CDS, c-myb13A_CDS, c-myb14A_CDS, c-myb8B_CDS, v-myb myeloblastosis viral oncogene homolog

Gene ID: 4602

NCBI Accession: NP_001123645, NP_005366, NP_001123644, NP_001155128, NP_001155129, NP_001155130, NP_001155131, NP_001155132

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process