Cadherin 5 antibody (PE)

Catalog No. ABIN5711933

This Mouse Monoclonal antibody specifically detects Cadherin 5 in FACS. It exhibits reactivity toward Human.

Quick Overview for Cadherin 5 antibody (PE) (ABIN5711933)

Target: Cadherin 5 (CDH5)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin 5 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 55-7H1

Product Details anti-Cadherin 5 Antibody (PE)

Purpose: Anti-Hu CD144 PE

Specificity: The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human endothelial cells

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: CD144

Background: Cadherin 5,CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.,Cadherin 5, CDH5, 7B4, VE-cadherin

Gene ID: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2