CIRBP antibody (AA 161-172)

Catalog No. ABIN571216

This Goat Polyclonal antibody specifically detects CIRBP in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for CIRBP antibody (AA 161-172) (ABIN571216)

Target: CIRBP (Cold Inducible RNA Binding Protein (CIRBP))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CIRBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CIRBP Antibody

Binding Specificity: AA 161-172

Purpose: CIRBP (aa 161-172)

Sequence: RDSYDSYATH NE

Specificity: This antibody is expected to recognize reported isoform 1 (NP_001271.1) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-RDSYDSYATHNE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining preferentially in type A spermatogonia. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 18 kDa band observed in Mouse Testis lysates (calculated MW of 18.6 kDa according to Human NP_001271.1 and Mouse NP_031731.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CIRBP

Target: CIRBP (Cold Inducible RNA Binding Protein (CIRBP))

Alternative Name: CIRBP

Background: CIRBP, cold inducible RNA binding protein, CIRP, Cold-inducible RNA-binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein

Gene ID: 1153, 12696, 81825

NCBI Accession: NP_001271

Pathways: Ribonucleoprotein Complex Subunit Organization