CD31 antibody (PE)

Catalog No. ABIN576414

This anti-CD31 antibody is a Mouse Monoclonal antibody detecting CD31 in FACS. Suitable for Human and Pig.

Quick Overview for CD31 antibody (PE) (ABIN576414)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Product Details anti-CD31 Antibody (PE)

Specificity: Recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutant protein binding studies. Immunoprecipitates a protein of ~130 kD from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.

Purification: Affinity purified

Immunogen: Porcine CD31/human IgGFc fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:1)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 100 μL of lysed whole blood or 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL distilled water.

Concentration: Lot specific

Buffer: Lyophilized, PBS, 0.09 % sodium azide, 5 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Lyophilized powder may be stored at 4°C before opening. DO NOT FREEZE! Aliquot and store at 4°C. Stable at 4°C as an undiluted liquid for 6 months. Dilute only prior to immediate use.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CAM-1 / CD31

Background: Name/Gene ID: PECAM1

Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA

Gene ID: 5175

Pathways: Regulation of Actin Filament Polymerization