Glutathione antibody

Catalog No. ABIN577695

This anti-Glutathione antibody is a Mouse Monoclonal antibody detecting Glutathione in WB, IP and IA. Suitable for .

Quick Overview for Glutathione antibody (ABIN577695)

Target: Glutathione

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutathione antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunoassay (IA)

Clone: L4H

Product Details anti-Glutathione Antibody

Brand: AbX™

Characteristics: Designed for Immunofluorescence and FACS Analysis
Supplied as 50 µg in PBS
For use under Non-Reducing conditions

Purification: Protein A Purified

Immunogen: Glutathione conjugated to Keyhole Limpet Hemocyanin

Isotype: IgG2a

Application Details

Application Notes: Western blotting, Immunoassay, and Immunoprecipitation
Western blotting, 1:1,000 151119 AbX™ Anti-Glutathione Mouse Monoclonal
Detection of protein-glutathione adducts on Western Blot under non-reducing conditions. PTP-1B refers to phosphotyrosine phosphatase 1B
HeLa cells incubated with Glutathione monoclonal, clone L4H, at a 1:100 dilution, counterstained with FITC- labeled goat anti-mouse IgG. Red nuclear stain is DAPI.

Comment:

Applications include Western blotting, Immunoassay and Immunoprecipitation Supplied in PBS with no carrier protein For use under Non-Reducing conditions

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

Storage Comment: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Target Details for Glutathione

Target: Glutathione

Target Type: Chemical

Background: Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ Glutathione Mouse Monoclonal Antibody is produced as a Protein A purified antibody. It will measure GSH-protein complexes under non-reducing conditions.