TPBG antibody (AA 31-355)

Catalog No. ABIN602074

This Sheep Polyclonal antibody specifically detects TPBG in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TPBG antibody (AA 31-355) (ABIN602074)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This TPBG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TPBG Antibody

Binding Specificity: AA 31-355

Specificity: Recognizes the human Oncofetal Antigen 5T4 at ~72kD. Shows ~20 % cross-reactivity with mouse 5T4. Species sequence Homology: Mouse (81 %), canine (85 %).

Purification: Immunoaffinity purified

Immunogen: Recombinant human 5T4 extracellular domain (aa31-355).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (3 - 10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well. Western Blot: 1 μg/mL. Immunohistochemistry: 3-10 μg/mL detects 5T4 in cells and tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for TPBG

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Alternative Name: TPBG / 5T4

Background: Name/Gene ID: TPBG

Synonyms: TPBG, 5T4, 5T4-AG, 5T4AG, 5T4 oncofetal antigen, M6P1, Trophoblast glycoprotein

Gene ID: 7162

UniProt: Q13641