BCAN antibody (AA 23-911)

Catalog No. ABIN602839

This Mouse Monoclonal antibody specifically detects BCAN in WB, ELISA, IP and Func. It exhibits reactivity toward Human.

Quick Overview for BCAN antibody (AA 23-911) (ABIN602839)

Target: BCAN (Brevican (BCAN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCAN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Product Details anti-BCAN Antibody

Binding Specificity: AA 23-911

Specificity: Recognizes human Brevican.

Purification: Protein G purified

Immunogen: NS0-derived rhBrevican. (aa 23 - 911).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Func, IP (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 μg/mL detection limit for rhBrevican is ~1 ng/well. Western Blot: 1-2 μg/mL detection limit for rhBrevican is ~10 ng/lane under non-reducing conditions. Immunoprecipitation: 25 μg/mL. Blocking of receptor-ligand interaction: ~0.01 to 0.05 μg/mL of this antibody will block 50 % of the binding of 1 μg/mL of Hyaluronan (132kD) to immobilized rhBrevican (100 μL of a 3 μg/mL solution was coated in each well) in a functional ELISA. At 1 μg/mL, this antibody will achieve 100 % blocking in the assay.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for BCAN

Target: BCAN (Brevican (BCAN))

Alternative Name: BCAN / Brevican

Background: Name/Gene ID: BCAN

Synonyms: BCAN, Brevican, BEHAB, CSPG7, Brevican core protein

Gene ID: 63827

UniProt: Q96GW7

Pathways: Glycosaminoglycan Metabolic Process