Calmodulin 1 antibody (C-Term)

Catalog No. ABIN605114

The Mouse Monoclonal anti-Calmodulin 1 antibody has been validated for WB, IHC, IP, IHC (p) and RIA. It is suitable to detect Calmodulin 1 in samples from Human, Rat, Mouse, Cow, Pig and Wheat.

Quick Overview for Calmodulin 1 antibody (C-Term) (ABIN605114)

Target: Calmodulin 1 (Calm1)

Reactivity: Human, Rat, Mouse, Cow, Pig, Wheat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calmodulin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-Calmodulin 1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes both Ca 2+-bound and Ca 2+-free calmodulin. Does not recognize bovine S100- alpha, S100-beta, troponin C or parvalbumin. Species cross-reactivity: rat, mouse, human, porcine and wheat germ. Wide cross-reactivity expected.

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to the 21 carboxy-terminal amino acids of bovine calmodulin, conjugated with thyroglobulin.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, RIA, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation and RIA. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates calmodulin from A431 RIPA cell lysate.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Calmodulin 1

Target: Calmodulin 1 (Calm1)

Alternative Name: Calmodulin

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction, BCR Signaling