beta Actin antibody (N-Term)

Catalog No. ABIN609124

This Mouse Monoclonal antibody specifically detects beta Actin in WB, IHC, IHC (p) and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for beta Actin antibody (N-Term) (ABIN609124)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Specificity: Recognizes beta-actin. Species cross-reactivity: Human, Mouse, Rat.

Purification: Immunoaffinity purified

Immunogen: N-terminal peptide slightly modified b-cytoplasmic actin, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys (KLH).

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Western Blot: 0.25-0.5 μg/mL. Immunohistochemistry (paraffin): 0.5-1 μg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1.2 % sodium acetate

Concentration: Lot specific

Buffer: Lyophilized from 1.2 % sodium acetate, 2 mg BSA, 0.01 mg sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB / Beta Actin

Background: Name/Gene ID: ACTB

Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction