B3GALNT1 antibody (AA 204-253)

Catalog No. ABIN6746248

This Rabbit Polyclonal antibody specifically detects B3GALNT1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey and Pig and has been mentioned in 3+ publications.

Quick Overview for B3GALNT1 antibody (AA 204-253) (ABIN6746248)

Target: B3GALNT1 (beta-1,3-N-Acetylgalactosaminyltransferase 1 (B3GALNT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B3GALNT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-B3GALNT1 Antibody

Binding Specificity: AA 204-253

Specificity: Rat B3GALNT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa204-253 of rat B3galnt1 (Q6AY39, NP_001013176). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Platypus (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-B3GALNT1 antibody (ABIN6746248)

Jayaraman, Murthy, Zhu, Curran, Xanthoudakis, Prives: "Identification of redox/repair protein Ref-1 as a potent activator of p53." in: Genes & development, Vol. 11, Issue 5, pp. 558-70, (1997) (PubMed).

Xanthoudakis, Miao, Curran: "The redox and DNA-repair activities of Ref-1 are encoded by nonoverlapping domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 1, pp. 23-7, (1994) (PubMed).

Xanthoudakis, Miao, Wang, Pan, Curran: "Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme." in: The EMBO journal, Vol. 11, Issue 9, pp. 3323-35, (1992) (PubMed).

Target Details for B3GALNT1

Target: B3GALNT1 (beta-1,3-N-Acetylgalactosaminyltransferase 1 (B3GALNT1))

Alternative Name: B3GALNT1

Background: Name/Gene ID: B3GALNT1

Synonyms: B3GALNT1, Beta3Gal-T3, Beta3GalT3, B3Gal-T3, Beta-1,3-GalTase 3, B3GALT3, Globoside synthase, GLCT3, GLOB, GalT3, Gb4Cer, P antigen synthase, p1, Beta-1,3-GalNAc-T1, Beta-3-Gx-T3, Brainiac1, P blood group globoside

Gene ID: 8706

NCBI Accession: NP_001013176

UniProt: O75752