Goat anti-Cat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN611916

Quick Overview for Goat anti-Cat IgG (Heavy & Light Chain) Antibody (HRP) (ABIN611916)

Target: IgG

Conjugate: HRP

Application: Immunoassay (IA)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Cat

Host: Goat

Characteristics: Goat anti-Cat IgG (H&L) - Affinity Pure, HRP Conjugate, lyophilized.

Purification: Affinity purified using solid phase cat IgG (H&L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Cat IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all all immunoassays applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:2,000 - 1:20,000 for ELISA/Western blot, 1:500 - 1:5,000 for Immunohistochemistry

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody