Aconitase 1 antibody (AA 1-170)

Catalog No. ABIN6136437

This anti-Aconitase 1 antibody is a Rabbit Polyclonal antibody detecting Aconitase 1 in WB. Suitable for Human.

Quick Overview for Aconitase 1 antibody (AA 1-170) (ABIN6136437)

Target: Aconitase 1 (ACO1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: AA 1-170

Sequence: MSNPFAHLAE PLDPVQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAIRNC DEFLVKKQDI ENILHWNVTQ HKNIEVPFKP ARVILQDFTG VPAVVDFAAM RDAVKKLGGD PEKINPVCPA DLVIDHSIQV DFNRRADSLQ KNQDLEFERN RERFEFLKWG SQAFHNMRII

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human IREB1 (NP_002188.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1

Background: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants,ACO1,ACONS,HEL60,IREB1,IREBP,IREBP1,IRP1,Epigenetics & Nuclear Signaling,Signal Transduction,Endocrine & Metabolism,Nucleotide metabolism,Cardiovascular,Blood,ACO1

Molecular Weight: 98 kDa

Gene ID: 48

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis