Splicing Factor 1 antibody (AA 1-280)

Catalog No. ABIN6147661

The Rabbit Polyclonal anti-Splicing Factor 1 antibody has been validated for WB. It is suitable to detect Splicing Factor 1 in samples from Human.

Quick Overview for Splicing Factor 1 antibody (AA 1-280) (ABIN6147661)

Target: Splicing Factor 1 (SF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Splicing Factor 1 Antibody

Binding Specificity: AA 1-280

Sequence: MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH NLITEMVALN PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1

Background: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.,SF1,BBP,D11S636,MBBP,ZCCHC25,ZFM1,ZNF162,Epigenetics & Nuclear Signaling,Transcription Factors,RNA Binding,SF1

Molecular Weight: 59 kDa/61 kDa/65 kDa/67 kDa/68 kDa/71 kDa

Gene ID: 7536

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location