ULK1 antibody (AA 400-499)

Catalog No. ABIN6149921

This Rabbit Polyclonal antibody specifically detects ULK1 in WB and IF. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for ULK1 antibody (AA 400-499) (ABIN6149921)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ULK1 Antibody

Binding Specificity: AA 400-499

Sequence: RTPSPSPPCS SSPSPSGRAG PFSSSRCGAS VPIPVPTQVQ NYQRIERNLQ SPTQFQTPRS SAIRRSGSTS PLGFARASPS PPAHAEHGGV LARKMSLGGG

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-499 of human ULK1 (NP_003556.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:1000,IF,1:10 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-ULK1 antibody (ABIN6149921)

Xu, Zhang, Liu, Wu, Qin, Huang: "Activation of the SphK1/ERK/p-ERK pathway promotes autophagy in colon cancer cells." in: Oncology letters, Vol. 15, Issue 6, pp. 9719-9724, (2018) (PubMed).

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULk1

Background: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1 via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR, however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation.,ULK1,ATG1,ATG1A,UNC51,Unc51.1,hATG1,ULk1,Signal Transduction,Kinase,Serine/threonine kinases,PI3K-Akt Signaling Pathway,Cell Biology & Developmental Biology,Autophagy,Endocrine & Metabolism,Mitochondrial metabolism,Mitophagy fission and fusion,Neuroscience,Cardiovascular,Heart,Regulator of mTOR complex function,Regulators,ULk1

Molecular Weight: 112 kDa

Gene ID: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy