TAG-72 / CA72.4 antibody (CF®488A)

Catalog No. ABIN6153855

This Mouse Monoclonal antibody specifically detects in FACS and IF. It exhibits reactivity toward Human, Dog, Cow and Rat.

Quick Overview for TAG-72 / CA72.4 antibody (CF®488A) (ABIN6153855)

Target: TAG-72 / CA72.4

Reactivity: Human, Dog, Cow, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B72-3 CA72-733

Product Details

Purpose: Mouse Monoclonal anti-TAG-72 / CA72.4 (B72.3 + CA72/733), CF488A Conjugate

Characteristics: This antibody recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: TAG-72 protein (B72.3 & CA72/733)

Isotype: IgG

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mLImmunofluorescence 1-2 μg/mL

Comment:

Jurkat cells, Breast or lung carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: TAG-72 / CA72.4

Background: CA72.4, Tumor associated glycoprotein 72

Molecular Weight: 220 kDa

Gene ID: 182875