TOP1MT antibody (CF®594)

Catalog No. ABIN6154275

This Mouse Monoclonal antibody specifically detects TOP1MT in IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for TOP1MT antibody (CF®594) (ABIN6154275)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOP1MT antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TOP1MT-488

Product Details anti-TOP1MT Antibody (CF®594)

Purpose: Mouse Monoclonal anti-Topoisomerase-I, MT (TOP1MT/488), CF594 Conjugate

Characteristics: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline  pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human TOP1MT protein

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

A431 cells. Heart, Skeletal muscle, brain or fetal liver.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Alternative Name: Topoisomerase-I, MT

Background: DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase

Molecular Weight: 70 kDa

Gene ID: 116447, 528574

UniProt: Q969P6