ADRP antibody (AA 249-376) (CF®405S)

Catalog No. ABIN6155695

The Mouse Monoclonal anti-ADRP antibody has been validated for WB, IF and FACS. It is suitable to detect ADRP in samples from Human.

Quick Overview for ADRP antibody (AA 249-376) (CF®405S) (ABIN6155695)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADRP antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ADFP-1365

Product Details anti-ADRP Antibody (CF®405S)

Binding Specificity: AA 249-376

Purpose: Mouse Monoclonal anti-Adipophilin / Perilipin-2 (ADFP/1365), CF405S Conjugate

Characteristics: This antibody recognizes a protein of 48 kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Adipophilin protein fragment (aa249-376) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Alternative Name: Adipophilin / Perilipin-2

Background: Adipophilin, ADFP, Adipose differentiation-related protein (ADRP), Perilipin-2 (PLIN2)

Molecular Weight: 48 kDa

Gene ID: 123

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism