ADIPOQ antibody (CF®488A)

Catalog No. ABIN6156211

The Mouse Monoclonal anti-ADIPOQ antibody has been validated for ELISA, IHC, FACS, IF and Coat. It is suitable to detect ADIPOQ in samples from Human.

Quick Overview for ADIPOQ antibody (CF®488A) (ABIN6156211)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADIPOQ antibody is conjugated to CF®488A

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)

Clone: ADPN-1370

Product Details anti-ADIPOQ Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-Adiponectin (ADPN/1370), CF488A Conjugate

Characteristics: This MAb reacts with adiponectin, an adipocytokine. Adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Adiponectin protein fragment

Isotype: IgG2b

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• ELISA For coating
• use antibody without BSA
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa cells or Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin

Molecular Weight: 26-30 kDa

Gene ID: 9370