MILK FAT GLOBULIN antibody (CF®647)

Catalog No. ABIN6156425

This anti- antibody is a Mouse Monoclonal antibody detecting in IF, FACS and IHC (f). Suitable for Human.

Quick Overview for MILK FAT GLOBULIN antibody (CF®647) (ABIN6156425)

Target: MILK FAT GLOBULIN

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: EDM45

Product Details

Purpose: Mouse Monoclonal anti-Milk-Fat-Globulin (EDM45), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Delipidated human milk fat globule membrane preparation

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Normal breast or breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: MILK FAT GLOBULIN

Alternative Name: Milk-Fat-Globulin

Background: Breast epithelial antigen BA46, EDIL1, HMFG, Lactadherin, Lactadherin precursor, Medin, MFGE8, MFGM, Milk fat globule-EGF factor 8 (MFG-E8)

Molecular Weight: 45 kDa

Gene ID: 4240, 3745

UniProt: Q08431