Plasma Cell Marker antibody (Biotin)

Catalog No. ABIN6156598

The Mouse Monoclonal anti-Plasma Cell Marker antibody has been validated for IHC (f). It is suitable to detect Plasma Cell Marker in samples from Human.

Quick Overview for Plasma Cell Marker antibody (Biotin) (ABIN6156598)

Target: Plasma Cell Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: LIV3G11-same as 7B18

Product Details

Purpose: Mouse Monoclonal anti-Plasma Cell Marker (LIV3G11 (7B18)), Biotin Conjugate

Characteristics: This antibody recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4 lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm, their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This MAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this MAb is not suitable for staining frozen tissues. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Pancreatic cancer-related mucin

Isotype: IgG2a

Application Details

Application Notes: Does not react with rat, others not known

• Immunohistology formalin-fixed 0.1-0.2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Comment:

Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: Plasma Cell Marker

Background: Plasma B cell, Plasmocytes