CD28 antibody (CF®640R)

Catalog No. ABIN6158511

This Mouse Monoclonal antibody specifically detects CD28 in FACS and IF. It exhibits reactivity toward Human, Mouse, Cow, Pig and Sheep.

Quick Overview for CD28 antibody (CF®640R) (ABIN6158511)

Target: CD28

Reactivity: Human, Mouse, Cow, Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to CF®640R

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 204-12

Product Details anti-CD28 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-CD28 (204.12), CF640R Conjugate

Characteristics: This antibody recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leucocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mLFlow Cytometry 0.5-1 μg/million cells/0.1 mL

Comment:

Jurkat cells or human lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: T-cell-specific surface glycoprotein CD28, Tp44

Molecular Weight: 44 kDa (monomer), 88 kDa (dimer)

Gene ID: 940, 591629

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response