CD6 antibody (CF®647)

Catalog No. ABIN6160111

The Mouse Monoclonal anti-CD6 antibody has been validated for FACS, IF and IHC (f). It is suitable to detect CD6 in samples from Human.

Quick Overview for CD6 antibody (CF®647) (ABIN6160111)

Target: CD6

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD6 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C6-372 3F7B5

Product Details anti-CD6 Antibody (CF®647)

Purpose: Mouse Monoclonal anti-CD6 (C6/372 + 3F7B5), CF647 Conjugate

Characteristics: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular ?scavenger receptor cysteine-rich? (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human recombinant CD6 protein (C6/372), Human rheumatoid synovial T cell line ST-1 (3F7B5)

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

CCRF-CEM, Jurkat cells, Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD6

Target: CD6

Alternative Name: CD6

Background: T12, TP120

Molecular Weight: 90-130 kDa

Gene ID: 923, 744366

UniProt: P30203