Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®647)

Catalog No. ABIN6161137

The Mouse Monoclonal anti- antibody has been validated for WB, FACS, IHC and IF. It is suitable to detect in samples from Human, Mouse and Rat.

Quick Overview for Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) antibody (CF®647) (ABIN6161137)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SPTBN2-1584

Product Details

Binding Specificity: AA 356-475

Purpose: Mouse Monoclonal anti-Spectrin-beta-III (SPTBN2/1584), CF647 Conjugate

Characteristics: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin ? and spectrin ? are present in erythrocytes, whereas spectrin ? II (also designated fodrin ?) and spectrin ? I (also designated fodrin ?) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ? III is highly homologous to both spectrin ? I and spectrin ? II. Spectrin ? III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SPTBN2 fragment (aa356-475) (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa cells. Pancreas or Liver.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Alternative Name: Spectrin-beta-III

Background: Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

Molecular Weight: 246 kDa

Gene ID: 6712, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis