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Clathrin, Light Chain (N-Term) antibody (CF®405S)

Reactivity: Human WB, IHC, FACS, IF Host: Mouse Monoclonal CLC-1421 CF®405S
Catalog No. ABIN6162991
  • Target
    Clathrin, Light Chain
    Binding Specificity
    N-Term
    Reactivity
    • 9
    • 1
    • 1
    Human
    Host
    • 8
    • 1
    Mouse
    Clonality
    • 8
    • 1
    Monoclonal
    Conjugate
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    CF®405S
    Application
    • 9
    • 9
    • 8
    • 8
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    Mouse Monoclonal anti-Clathrin, Light chain (CLC/1421), CF405S Conjugate
    Characteristics
    This antibody recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Purified recombinant N-terminal fragment of human Clathrin Light Chain
    Clone
    CLC-1421
    Isotype
    IgG2b kappa
  • Application Notes
    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    HeLa cells. Placenta or Prostate Carcinoma.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Clathrin, Light Chain
    Molecular Weight
    31-44 kDa
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