CD8 antibody (C-Term, Cytoplasmic Domain)

Catalog No. ABIN6165573

This anti-CD8 antibody is a Mouse Monoclonal antibody detecting CD8 in FACS, IF and IHC (f). Suitable for Human.

Quick Overview for CD8 antibody (C-Term, Cytoplasmic Domain) (ABIN6165573)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C8-144B

Product Details anti-CD8 Antibody

Binding Specificity: C-Term, Cytoplasmic Domain

Purpose: Mouse Monoclonal anti-CD8 (C8/144B), Purified, with BSA

Characteristics: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 α/β) or as a homodimer (CD8 α/α). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: A 13 amino acid peptide from C-terminal cytoplasmic domain of alpha chain of human CD8 molecule.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

HuT78 or hPBL. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

Molecular Weight: 32 kDa

Gene ID: 925, 85258

UniProt: P01732