Moesin antibody (Biotin)

Catalog No. ABIN6165718

The Mouse Monoclonal anti-Moesin antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect Moesin in samples from Human.

Quick Overview for Moesin antibody (Biotin) (ABIN6165718)

Target: Moesin (MSN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MSN492

Product Details anti-Moesin Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Moesin (MSN492), Biotin Conjugate

Characteristics: This antibody recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Moesin protein

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with rat, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HT-29, CH3LC, or HUVEC cells. Uterus, Placenta, Tonsil, Melanoma, Testicular Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: Moesin

Background: Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSN/ALK fusion

Molecular Weight: 78 kDa

Gene ID: 4478, 87752

UniProt: P26038

Pathways: Asymmetric Protein Localization