hCG Intact antibody (CF®640R)

Catalog No. ABIN6165769

The Mouse Monoclonal anti-hCG Intact antibody has been validated for IHC (f). It is suitable to detect hCG Intact in samples from Human.

Quick Overview for hCG Intact antibody (CF®640R) (ABIN6165769)

Target: hCG Intact

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®640R

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HCGab-52

Product Details

Purpose: Mouse Monoclonal anti-HCG intact (HCGab/52), CF640R Conjugate

Characteristics: This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the ? ? and ? ? subunits. The ? ? subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified hCG protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Comment:

JAR or TT Cells. Placenta

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: hCG Intact

Molecular Weight: ~35 kDa (intact hCG)

Gene ID: 1081, 1082