NAPSA antibody (Biotin)

Catalog No. ABIN6165984

This Mouse Monoclonal antibody specifically detects NAPSA in WB, IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for NAPSA antibody (Biotin) (ABIN6165984)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPSA antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NAPSA-1238

Product Details anti-NAPSA Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Napsin-A (NAPSA/1238), Biotin Conjugate

Characteristics: Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Napsin-A protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 1-2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Lung adenocarcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: Napsin-A

Background: ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Molecular Weight: 37 kDa

Gene ID: 9476, 512843

UniProt: O96009

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis