PAX8 antibody (AA 60-261) (CF®405S)

Catalog No. ABIN6166183

The Mouse Monoclonal anti-PAX8 antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect PAX8 in samples from Human.

Quick Overview for PAX8 antibody (AA 60-261) (CF®405S) (ABIN6166183)

Target: PAX8 (Paired Box 8 (PAX8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX8 antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PAX8-1492

Product Details anti-PAX8 Antibody (CF®405S)

Binding Specificity: AA 60-261

Purpose: Mouse Monoclonal anti-PAX8 (PAX8/1492), CF405S Conjugate

Characteristics: This antibody recognizes a protein of 62 kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human PAX8 fragment (aa60-261) (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

•   pH 9.0
• for 10-20 min followed by cooling at RT for 20 min
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Raji cells. Renal Cell Carcinoma (RCC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: Paired box 8, Paired box gene 8, paired box homeotic gene 8, Paired box protein Pax-8, Paired domain gene 8, PAX8

Molecular Weight: 62 kDa

Gene ID: 7849, 469728

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation