alpha Fetoprotein antibody (Biotin)

Catalog No. ABIN6168513

This Mouse Monoclonal antibody specifically detects alpha Fetoprotein in IF, FACS and IHC (f). It exhibits reactivity toward Human, Pig, Dog and Monkey.

Quick Overview for alpha Fetoprotein antibody (Biotin) (ABIN6168513)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Pig, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C3

Product Details anti-alpha Fetoprotein Antibody (Biotin)

Purpose: Mouse Monoclonal anti-AFP (Alpha-Fetoprotein) (C3), Biotin Conjugate

Characteristics: This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL

• Does not react with cow, dog, mouse, or rat
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP (Alpha-Fetoprotein)

Background: Alpha fetoglobulin, FETA, HPAFP

Molecular Weight: 70 kDa

Gene ID: 174, 518808

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process