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EPO antibody (Erythropoietin) (AA 28-162) (CF®488A) Primary Antibody

EPO Reactivity: Human FACS, IF, IHC, WB Host: Mouse Monoclonal EPO-1368 CF®488A
Catalog No. ABIN6174282
$270.77
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  • Target See all EPO Antibodies
    EPO
    Binding Specificity
    • 39
    • 16
    • 12
    • 11
    • 10
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 28-162
    Reactivity
    • 155
    • 33
    • 32
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 109
    • 68
    • 3
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 114
    • 70
    Monoclonal
    Conjugate
    • 96
    • 19
    • 11
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EPO antibody is conjugated to CF®488A
    Application
    • 124
    • 85
    • 62
    • 46
    • 44
    • 14
    • 13
    • 13
    • 10
    • 8
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Purpose
    Mouse Monoclonal anti-Erythropoietin (EPO/1368), CF488A Conjugate
    Characteristics
    This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant fragment of human EPO protein (aa28-162) (exact sequence is proprietary)
    Clone
    EPO-1368
    Isotype
    IgG
  • Application Notes
    Immunohistology (formalin) 4-8 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    HepG2 Cells. Heart or Kidney.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    EPO
    Alternative Name
    Erythropoietin (EPO Products)
    Synonyms
    EPO, EP, MVCD2, erythropoietin, erythropoietin S homeolog, erythropoietin a, EPO, epo, epo.S, Epo, epoa
    Target Type
    Hormone
    Background
    EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2
    Molecular Weight
    37 kDa
    Gene ID
    2056, 2303
    UniProt
    P01588
    Pathways
    JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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