ABO (Blood Group Antigen B) antibody

Catalog No. ABIN6182163

This Mouse Monoclonal antibody specifically detects in IHC, H, IF and AGG. It exhibits reactivity toward Human.

Quick Overview for ABO (Blood Group Antigen B) antibody (ABIN6182163)

Target: ABO (Blood Group Antigen B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Haemagglutination (H), Immunofluorescence (IF), Agglutination (AGG)

Clone: HEB-29

Product Details

Purpose: Mouse Monoclonal anti-Blood-Group-B (HEB-29), Purified, BSA-free

Characteristics: The antibody HEB-29 reacts with human blood group B. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. MAb HEB-29 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. It is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B

Isotype: IgM kappa

Application Details

Application Notes: Agglutination

• Immunohistology (formalin)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

KG1 cells or human colorectal carcinoma tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details

Target: ABO (Blood Group Antigen B)

Alternative Name: Blood-Group-B

Molecular Weight: Multiple

Gene ID: 28, 654423

UniProt: P16442