Cadherin-16 antibody

Catalog No. ABIN6182923

The Mouse Monoclonal anti-Cadherin-16 antibody has been validated for IF, FACS and IHC (f). It is suitable to detect Cadherin-16 in samples from Human, Mouse, Dog and Rabbit.

Quick Overview for Cadherin-16 antibody (ABIN6182923)

Target: Cadherin-16 (CDH16)

Reactivity: Human, Mouse, Dog, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CDH16-1071

Product Details anti-Cadherin-16 Antibody

Purpose: Mouse Monoclonal anti-Ksp-Cadherin / CDH16 (CDH16/1071), Purified, BSA-free

Characteristics: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human CDH16 protein

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Normal kidney or renal cell carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: Ksp-Cadherin / CDH16

Background: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody

Molecular Weight: 130 kDa

Gene ID: 1014, 513660

UniProt: O75309