CD235a/GYPA antibody

Catalog No. ABIN6184671

This Mouse Monoclonal antibody specifically detects CD235a/GYPA in FACS, IF and AGG. It exhibits reactivity toward Human and Cow.

Quick Overview for CD235a/GYPA antibody (ABIN6184671)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Agglutination (AGG)

Clone: GPHN02

Product Details anti-CD235a/GYPA Antibody

Purpose: Mouse Monoclonal anti-Glycophorin-A / CD235a (GPHN02), Purified, BSA-free

Characteristics: This antibody recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Human erythrocytes treated with neuraminidase

Isotype: IgM kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Erythrocyte Agglutination
• Optimal dilution for a specific application should be determined by user

Comment:

Erythrocytes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: Glycophorin-A / CD235a

Background: Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha

Molecular Weight: 39 kDa

Gene ID: 2993, 2994

UniProt: P02724

Pathways: Maintenance of Protein Location